[flick]

How do u do the following haven't got a clue how to do these:confused

Glucose micrograms/ml absorbance 620nm
0 0.035; 0.028
10 0.085; 0.091
30 0.185; 0.191
60 0.335; 0.342
90 0.480; 0.478
1ml hydrolysate 0.275; 0.266

(I) plot these data and establish a standrad curve for glucose
(ii) determine the glucose content of the final hydrolysate
(iii) determine the glucose content of the original sample
(iv) establish the percentage hydrolysis of glycogen

[templelane]

Here is a partial hand to help you on your way

If you plot micrograms/ml of glucose (first column) against mg absorbance (second and third column- average these) on a graph. This is your standard curve.

Right I won't give you all the answers as once you have this the rest should become clear- post back if you are still stuck/ confused and I'll help you will the rest.