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-   -   Biochemistry standard curve for glucose (https://www.askmehelpdesk.com/showthread.php?t=115525)

  • Aug 1, 2007, 12:18 PM
    flick
    Biochemistry standard curve for glucose
    How do u do the following haven't got a clue how to do these:confused

    Glucose micrograms/ml absorbance 620nm
    0 0.035; 0.028
    10 0.085; 0.091
    30 0.185; 0.191
    60 0.335; 0.342
    90 0.480; 0.478
    1ml hydrolysate 0.275; 0.266

    (I) plot these data and establish a standrad curve for glucose
    (ii) determine the glucose content of the final hydrolysate
    (iii) determine the glucose content of the original sample
    (iv) establish the percentage hydrolysis of glycogen
  • Aug 1, 2007, 12:26 PM
    templelane
    Here is a partial hand to help you on your way

    If you plot micrograms/ml of glucose (first column) against mg absorbance (second and third column- average these) on a graph. This is your standard curve.

    Right I won't give you all the answers as once you have this the rest should become clear- post back if you are still stuck/ confused and I'll help you will the rest.

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