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        For gasses there is a relationship between moles and volume, known as the ideal gas law.  One mole of an ideal gas at standard temperature and pressure ("STP" = 0 degrees C, 1 atmosphere pressure) occupies 22.4 liters of volume.  Thus 100 liters of an ideal gas at STP is 100/22.4 = 4.46 moles.  Multiply by Avogadro's number to get the number of molecules: 4.46 moles x 6.022 x 10^23 molecules/mole = 2.69 x 10^24 molecules.
 If the temp and pressure are not STP, you can use the ideal gas law to determine how many moles there are in a given volume:  PV = nRT, where P=pressure(Pa), V = volume (m^3), n = number of moles, R = gas constant, and T = temp in Kelvins.  The value for R depends on the units you're using - two of the most common are:
 
 R= 8.314J/Mol K = 8.314 Pa m^3/Mol K, and
 R = 0.082057 L Atm/mol K.
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