Hi tsullivan,
It could be due to a number of possibilities. Changes in hormone levels due to age perhaps, or it could be spotting from recent intercourse. Some women who use hormonal forms of birth control may have spotting or bleeding at various times of their cycle. Some women will experience some spotting around the time of ovulation. Those are just a few possible causes. It can happen where you just don't have an explanation and then the next month everything is back to normal.
See what happens when your next period is due. If it doesn't show after several days of when you would expect, then try a pregnancy test.
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