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    Graceyel's Avatar
    Graceyel Posts: 6, Reputation: 1
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    #1

    Aug 22, 2012, 06:36 AM
    Converting millimoles to microMoles
    I know you multiply by 1000 to get umol to mM however what do you do with extra units ? For example:
    Umol min-1 mL-1 to mM
    Would it just become mM min-1 mL-1?
    ebaines's Avatar
    ebaines Posts: 12,131, Reputation: 1307
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    #2

    Aug 22, 2012, 07:01 AM
    Actually you divide micromoles by 1000 to get units of millimoles. Starting with

    X uMol/(Min-mL)

    multiply by 1 \mMol/(1000 uMol) to get:

    X uMol/(Min-mL) x (1 mMol}/(1000 uMol} = (X/1000) mMol/(min-mL)
    Graceyel's Avatar
    Graceyel Posts: 6, Reputation: 1
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    #3

    Aug 22, 2012, 07:37 AM
    Sorry I meant micromoles to millimoles. For example 0.0022 umol min-1 mL-1 to mM
    Thank you so much for your help
    Graceyel's Avatar
    Graceyel Posts: 6, Reputation: 1
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    #4

    Aug 22, 2012, 07:46 AM
    Quote Originally Posted by ebaines View Post
    Actually you divide micromoles by 1000 to get units of millimoles. Starting with

    X uMol/(Min-mL)

    multiply by 1 \mMol/(1000 uMol) to get:

    X uMol/(Min-mL) x (1 mMol}/(1000 uMol} = (X/1000) mMol/(min-mL)
    Sorry I meant micromoles to millimoles. For example 0.0022 umol min-1 mL-1 to mM
    Thank you so much for your help
    Graceyel's Avatar
    Graceyel Posts: 6, Reputation: 1
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    #5

    Aug 22, 2012, 08:24 AM
    Convert 0.0023 umol min-1 mL-1 to mM? Lineweaver Burke plot
    Convert 0.0022 umol min-1 mL-1 to mM?

    This is for a lineweaver Burke plot. I have a table as follows:
    Substrate (mM) No inhibitor. Inhibitor
    Vo(umol min-1 mL-1)
    0.05. 0.0022. 0.0045
    0.1. 0.0033. 0.0083
    0.2. 0.044. 0.0154
    0.5. 0.059. 0.0286
    2. 0.063. 0.05

    Am I best to convert the Vo(umol min-1 mL-1) to mM to plot the graph? If so any help on how to convert these would be greatly appreciated!!
    I know afterwards I need to do 1/[S] and 1/velocity afterwards but I'm just stuck here.
    Thank you in advance!
    Graceyel's Avatar
    Graceyel Posts: 6, Reputation: 1
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    #6

    Aug 22, 2012, 11:35 AM
    Lineweaver burk plot
    Please will someone help! I don't understand how to get the data to plot. As the 1/v would give 454 which doesn't seem right ?
    For example substrate is 0.5 mM and velocity 0.0022 umol min-1 mL-1. Conversion factor 1 so 0.0022 mm-1 min. I just don't know what else to do with 0.0022?? And the rest on the graph! Any help appreciated

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