I know you multiply by 1000 to get umol to mM however what do you do with extra units ? For example:
Umol min-1 mL-1 to mM
Would it just become mM min-1 mL-1?

Actually you divide micromoles by 1000 to get units of millimoles. Starting with

X uMol/(Min-mL)

multiply by 1 \mMol/(1000 uMol) to get:

X uMol/(Min-mL) x (1 mMol}/(1000 uMol} = (X/1000) mMol/(min-mL)

Sorry I meant micromoles to millimoles. For example 0.0022 umol min-1 mL-1 to mM
Thank you so much for your help

Quote:

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Originally Posted by ebaines

Actually you divide micromoles by 1000 to get units of millimoles. Starting with

X uMol/(Min-mL)

multiply by 1 \mMol/(1000 uMol) to get:

X uMol/(Min-mL) x (1 mMol}/(1000 uMol} = (X/1000) mMol/(min-mL)

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Sorry I meant micromoles to millimoles. For example 0.0022 umol min-1 mL-1 to mM
Thank you so much for your help

Convert 0.0022 umol min-1 mL-1 to mM?

This is for a lineweaver Burke plot. I have a table as follows:
Substrate (mM) No inhibitor. Inhibitor
Vo(umol min-1 mL-1)
0.05. 0.0022. 0.0045
0.1. 0.0033. 0.0083
0.2. 0.044. 0.0154
0.5. 0.059. 0.0286
2. 0.063. 0.05

Am I best to convert the Vo(umol min-1 mL-1) to mM to plot the graph? If so any help on how to convert these would be greatly appreciated!!
I know afterwards I need to do 1/[S] and 1/velocity afterwards but I'm just stuck here.
Thank you in advance!

Please will someone help! I don't understand how to get the data to plot. As the 1/v would give 454 which doesn't seem right ?
For example substrate is 0.5 mM and velocity 0.0022 umol min-1 mL-1. Conversion factor 1 so 0.0022 mm-1 min. I just don't know what else to do with 0.0022?? And the rest on the graph! Any help appreciated