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    Harst's Avatar
    Harst Posts: 3, Reputation: 1
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    #1

    Dec 29, 2010, 07:59 PM
    How to know the content of beta carotene in a solution?
    Hi all... is there any way to know the content of beta carotene in a solution?

    Form of beta carotene is C40H56...

    Whether there are elements that can react with beta-carotene in solution so that can change the color of the solution?

    Is anyone here know about that? Help me please... thanks
    DrBob1's Avatar
    DrBob1 Posts: 425, Reputation: 86
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    #2

    Dec 29, 2010, 09:22 PM
    There are many reagents that will react with the double bonds in beta-carotene. Many of them are colored (I2, Br2, KMnO4) and will be declolorized upon reaction. They also will decolorize the beta-carotene itself but I don't think this could be used quantitatively since the color of beta-carotene comes from its long conjugated system of double bonds. Fewer double bonds or an interrupted conjugated system would also change the color. I think you would be better served with UV/VIS spectroscopy. Hydrogenation to completion would be another possibility. You can also compare the color of known solutions with a solution of unknown concentration. Does this help?
    Harst's Avatar
    Harst Posts: 3, Reputation: 1
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    #3

    Jan 10, 2011, 08:37 PM
    Comment on DrBob1's post
    Thanks a lot DrBob1 for the answer... which one is the cheapest between I2, Br2, KMnO4?
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