The company I work for was recently acquired by another company. As a result, we no longer will be using Fidelity as our investment company. Can I take a cash withdrawl of all my my funds in the Fidelity fund?

First of course you don't want to withdraw it, since you will have to pay large amount of taxes and penalty.

I anticipated a tax penalty, and under normal circumstances I would roll it over. However, due to an unforseen problem, I would like to take it out in cash. So, would I be allowed to cash out?

First, it matters what type of retirement fund. Second, Is the current plan being terminated or will the funds simply be transferred to the new company's plan?

So we need more info here.