Their culturing and maintenance. Characterization of cultured cells, test of viability, cytotoxicity and measurement of growth.primary cultures, anchorage dependent growth.non - anchorage dependent cells. Secondary cultures. Commonly used animal cell lines- their origin and characteristics.

Either by passaging/subculturing the cell line after reaching 70-80%confluent if they are growing as an monolayer on a flask(T25) which provide an support for the cell to attach or storing them in the presence of cryopreservative agent(DMSO) at low temperature in liquid nitrogen.