Can you please tell me how or what would be the reaction if you 10% of H2SO4on a test tube with RNA?

BENEDICTS'S TEST
On the first experiment, solid Na2CO3 will be place on the test tube with 10% of H2SO4 and RNA on it. Would you please tell me what would be the reaction that will happen?

TEST FOR PURINE
On the second experiment, 1% AgNO3 will be placed on a test tube that has the given 10% H2SO4 and RNA on it. What would be the reaction?

TEST FOR PENTOSE
On the third experiment, 5ml of Bial's reagent will be placed on a test tube with 10% of H2SO4 and RNA on it. What would be the reaction?

TEST FOR PHOSPHATE
On the fourth experiment, 5 drops of ammonia water that is acidify with 2 drops of 6N HNO3 and (NH4)2 MoO4, it will be placed on a hot water bath for minutes. What would be the reaction?


*The RNA used is the Isolated RNA from yeast.


Please also the rationales on why those things happen.

Thanks. I need it so badly. :)

What is the result of NA2CO3 with benedict's solution

Guys, can you please answer this question? I also need it!

Benedict's test: brick red ppt
Orcinol test: bluish green sol'n

I got the same set of tests/procedure... dont know the answers yet

CONCLUSION
• Acid hydrolysis of DNA extracts trace amounts of pentoses (deoxyribose), purine bases (A & G), and proteins from DNA = good way to separate DNA into its components
• Base hydrolysis of DNA cannot extract any of the components being tested.
• Unhydrolyzed DNA : negative for all except for Xanthoproteic test and test for inorganic Pi
• Acid hydrolysis of RNA extracts trace amounts of reducing sugars, pentoses, purine bases, proteins, and phosphoric acid.
• Base hydrolysis of RNA extracts pentoses, purine bases, proteins, and phosphoric acid.
• Unhydrolyzed RNA positive for all test except presence of C & T; and A & G = best way to separate RNA into its components.
• Sample extracted from yeast contains RNA, but impure.


2. Based on the qualitative tests, how can you differentiate hydrolyzed from unhydrolyzed RNA?

• Unhydrolyzed RNA: positive for all except for the presence of uracil and cytosine
 Pentoses, purine(A & G) and pyrimidine bases, proteins, and phosphoric acid were extracted
 No uracil
 Acid-hydrolyzed RNA:
 Trace amounts of reducing sugars, pentoses, purine(A & G) and pyrimidine bases, Phe, Tyr, Trp, and phosphoric acid
 No tri- and polypeptides
 Base-hydrolyzed RNA
 pentoses, purine(A & G) and pyrimidine bases, tri- and polypeptides, Phe, Tyr, Trp, and phosphoric acid
 0.05% NaOH is the best reagent to separate RNA into its components

3. Based on these experimental results, do you think you obtained pure RNA from yeast? Why or why not?
• We obtained impure RNA from yeast
 Pentoses present : RNA and/or DNA
 Deoxyribose not present : RNA
 Uracil and Thymine : not present
 Purine bases are present
 Presence of tri- and polypeptides = impurities