So I am pregnant. I am considering a cvs test.. because I had sex with two partners 5 days apart. One for sure was using protection but there is a chance that he may have taken it off for a little while. He did not release inside of me but because he was in without the condom on for any point in time I feel like I can't be too sure.

The one who I know released in me is my long time partner of 4 years. We have been off and on for sometime. I am sure he released and from calculations I was with him at the very end of my ovulation. And I am leaning mor towards him being the father since I know he released and it was at the end of my ovulation period. However, my interaction with my other partner was five days before that so technically that would have been at the beginning of ovulation period. But without him releasing inside it would make a slimmer chance in my unprofessional opinion for him to be the father.

My question is... is it possible to tell by hormone levels or some other sort of levels to isolate which one is the father without going through the CVS test? I know the exact dates I was with each and I know the date of my lmp.(last missed period). Since I am a new mother and the doctors appointments are going to be all special moments I don't want to have the wrong man present.

The CVS test is merely to determine if there are possible health concerns with the baby. The only way to know for sure which one is the father would be to do a paternity test. Although not conclusive, odds are it is your long term boyfriend for two reasons. One, the other guy used a condom (at least part of the time) and did not release in you... and two it was 5 days before suspected ovulation. If your calculations are correct, his sperm may not have lived long enough to do the job. While some sperm may live 5 or so days, the majority will die off within 2 or 3. Since your boyfriend did release in you and it was closer to your ovulation, odds increase in that instance.
You'll have to wait to know for sure I'm afraid.

There is no way to determine level or age to within 5 days.
You will really need to wait till the child is born and have a DNA test done to determine who the father is. But yes if this was Vegas odds are with the LTB

If you can give me the first day of your last period, I can most likely tell you the date on which you conceived.

Aug 3 and my cycles are regular so my next menstrual cycle should have started on the 31/1st Sept. Counting 14 days back from my missed period lands me directly on the date I was with my LTB.

Okay, looking at my handy dandy gestational wheel that we use in my hospital, with your period starting on August 3, you most likely conceived on August 17 and your due date is May 8.

I hope this narrows it down for you.