Hi,
I am 28 yrs married on feb2006, wife's age 21
From more than one year we are not using any contraceptives
But she is not pregnant
She is under treatment of General Physician
2 times Ovaluation study done
But pregnancy not occurred
She have regular periods
What will be possible root cause
Please advise

It can take a long time for some women to conceive. You say she has been seen by a dr, have you? It could be a male problem not necessarily a female one. However, it may well just be you are both trying too hard. Just relax about having a baby and see what happens.