I am in the middle of a biology course I did an experiment last week and have forgotten the answer when mixing liver enzymes with water why is there no reaction:rolleyes:

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Originally Posted by akiko

i am in the middle of a biology course i did an experiment last week and have forgotten the answer when mixing liver enzymes with water why is there no reaction:rolleyes:

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You need energy. NADPH regenerating reaction. Plus Phosphate Reaction Buffer.
What was the reaction you were hoping for? Did you need to oxidize something?

Well liver enzymes... are protiens,, and protiens are insoluble in water, so most probably no reaction would take place..
OK .

But you see enzymes are usually basic .so just what will happen is that it will make a dilution

Well you see ,u need to specify here that the liver enzyme you are going to solubilize are in what state are they
I mean if they are extracted n r in most stable form
Because usually they are mixed in buffer and are at maintained temperature to arrest the activity... so you need to specify a bit more.


Well most aparently noreaction would take place from your statement quoted, if correct>>...

The liver enzyme that you were most likely working with (which is also present in potatoes) is "catalase." Catalase is a "neutral" working enzyme (works best at a pH level of 7). It converts hydrogen peroxide H202, into a water molecule (H20) and a hydrogen atom. So the point that your prof was most likely getting across was that catalase will do nothing when introduced to water because the substrate has already been broken down.

Sorry, It converts hydrogen peroxide H202, into a water molecule (H20) and an oxygen atom.**** my bad