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% Carbondioxide calculation using handerson hasselbach equation
With values of pH, Nahco3 concentration and metres above sea level available, how do we arrive at % CO2 based on handerson hasselbach equation? I have a carbondioxide incubator- culture media Iam placing in the incubator should have a pH of 7.2, NaHco3 concentration in the media is 25mM and my lab is located 435metres above sea level. What percentage of carbondioxide should the incubator be set at to derive this pH?
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