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    Gernald's Avatar
    Gernald Posts: 901, Reputation: 93
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    #1

    Nov 16, 2009, 03:07 PM
    Reading glycoproteins?
    Ekkk... okay...

    So does anyone out there in cyber space know how to determine what a glycoprotein is? I have what I guess is a western blot that has two parts conA-AMM control group and just a conA group. Do the glycoproteins stick to both or do they stick to just the connA group?
    jem02081's Avatar
    jem02081 Posts: 65, Reputation: 19
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    #2

    Nov 16, 2009, 07:18 PM
    ConA is a lectin. It binds to specific kinds of sugars that are found on proteins.
    You can detect protein which contain these sugars (glycoproteins) by ConA binding to them (ex in a Western Blot)
    AMM is alpha methyl mannoside. This is sugar that binds to ConA.
    Enough said?
    Gernald's Avatar
    Gernald Posts: 901, Reputation: 93
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    #3

    Nov 16, 2009, 08:40 PM

    So if I'm looking for glycoproteins in a western blot they'll show up on just the ConA-AMM and the other sticky proteins won't?
    jem02081's Avatar
    jem02081 Posts: 65, Reputation: 19
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    #4

    Nov 16, 2009, 08:57 PM

    No, the specific binding will show up with the ConA & the addition of AMM will block this.
    The AMM will block all of the ConA binding that's lectin specific & leave you with the NSB. So ConA-AMM will detect the sticky proteins only.
    Gernald's Avatar
    Gernald Posts: 901, Reputation: 93
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    #5

    Nov 16, 2009, 10:01 PM
    :-(
    Great now I have to re-do my homework.

    Just to clarify w/ the pict below, the one on the left shows sticky proteins while the one on the right shows both.
    Thanks so much for your help!
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    jem02081's Avatar
    jem02081 Posts: 65, Reputation: 19
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    #6

    Nov 16, 2009, 10:10 PM

    Right!
    Gernald's Avatar
    Gernald Posts: 901, Reputation: 93
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    #7

    Nov 16, 2009, 10:19 PM

    Thank you, thank you, thank you!!
    (I'd give you one of those cool green things, but it won't let me!)
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