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    ewsame Posts: 13, Reputation: 1
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    #1

    Apr 10, 2009, 09:28 AM
    Questions regarding biostatistics
    I am doing biological research and having some questions.

    I have 4 different groups of transgenic mice (A-D) and want to compare tumor sizes each other (group by group).

    A=control (wild type), n=3 mice
    B=gene B overexpressed, n=4 mice
    C=gene C deleted, n=4 mice
    D=gene B overexpressed & gene C deleted, n=3 mice

    Hypothesis of this study is that overexpression of gene B or deletion of gene C may induce tumor (by increasing tissue weight), and both may synergistically induce tumorigenisis.

    I sacrificed them, took tissues and measured their weights.

    For example, their weights are as followed,
    (unit=gram)

    A= 0.3/0.4/0.5
    B= 0.6/0.7/0.8/0.6
    C=0/7/0.8/0.7/0.6
    D=1.1/1.2/1.2

    First I want to calculate average/mean (dont know what is correct term in this case).

    Second I want to compare them group by group by calculating p values to see whether the difference is significant (our hyp is correct or not).
    group A vs B
    group A vs C
    group B vs D
    group C vs D
    In short, I want to see if B/C is heavier than A and D is heavier than B/C. (I will say their differences are significant/our hyp is correct if p<0.05)

    Now questions are followed.

    1. Do I have to calculate average or mean?
    2. What Test do I have to use to get p values?
    3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? Standard error(SE) OR standard deviation(SD)?

    Thank you su much in advance!
    ewsame's Avatar
    ewsame Posts: 13, Reputation: 1
    New Member
     
    #2

    Apr 10, 2009, 09:31 AM
    Thank you so much in advance!

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