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    mekran12's Avatar
    mekran12 Posts: 22, Reputation: 1
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    #1

    Nov 13, 2005, 04:36 PM
    Optimal temperature of Lactose
    What is the approximate optimal temperature that the enzyme lactose works best at to allow expression of beta-galactosidase in bacterial strains?
    kp2171's Avatar
    kp2171 Posts: 5,318, Reputation: 1612
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    #2

    Nov 21, 2005, 03:38 AM
    check supplier info.

    seen a lot of expmts run at 37C, but there's thermal resist enzyme that can be used at 65C, and others have run at room temp.

    if in doubt you could always do a quick study with temp as a variable... otherwise I'd talk to the manufacturer if you don't have the data on hand.
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