Ask Experts Questions for FREE Help !
Ask

Search:

Type: Posts; User: medgen

Search: Search took 0.00 seconds.

  1. Question: Human VS Pig

    by medgen
    Answers
    7
    Views
    23,120

    Actually, if they are mixed then you will be out...

    Actually, if they are mixed then you will be out of luck. Only if they are physically separate can you tell them apart.
  2. Answers
    6
    Views
    3,575

    Having made many liposomes in my time and at that...

    Having made many liposomes in my time and at that time working next to the lab of one of the pioneers in the field, I think I might be able to help (albeit belatedly).

    Liposomes refer to anything...
  3. Answers
    13
    Views
    16,276

    There is a very simple test we perform in our lab...

    There is a very simple test we perform in our lab that looks and works very much like a pregnancy test. You drop some of the diluted blood on the card -- two bands it's human, one band it is not. ...
  4. This would be exceptionally easy to do. The...

    This would be exceptionally easy to do. The semen would not need to be terribly fresh -- if it is in a condom it could probably be used for several days in this way. In our lab we generally get...
  5. So here I am again, still dealing with this... ...

    So here I am again, still dealing with this...

    About six months ago we were sent a letter by CBCL offering to make the debt go away if we paid a portion (about $400). I refused to do so on...
  6. Answers
    1
    Views
    1,929

    If iron is the catalyst for tests that detect...

    If iron is the catalyst for tests that detect presence of blood, what compounds might give a false positive test?

    Iron is not the catalyst in these tests, heme is. More specifically, the...
  7. Question: Storing Human DNA

    by medgen
    Answers
    6
    Views
    1,595

    Interesting comments from manimuth and Capuchin....

    Interesting comments from manimuth and Capuchin.

    Getting back to the original question, DNA is an incredibly stable molecule. It is most stable when stored dried (dessicated) and in a cool...
  8. Question: Animal cell basics

    by medgen
    Answers
    2
    Views
    1,041

    I'd say the study of proteins is really...

    I'd say the study of proteins is really biochemistry. Their interactions, kinetics, functions and structure are all studied in biochemistry. Proteomics is really a new field that looks at...
  9. Question: Biochem essays

    by medgen
    Answers
    3
    Views
    1,227

    It is a little foggy, but to most biochemists, a...

    It is a little foggy, but to most biochemists, a 'cell-based' assay would be in vivo (although to a macrobiologist this might be considered in vitro. Essentially (again, to a biochemist), in vitro...
  10. Answers
    5
    Views
    3,304

    I've done these assays a few times, and my...

    I've done these assays a few times, and my recollection is that phosphorylation usually causes a visible reduction in migration (i.e. band appears larger) on SDS-PAGE. Phosphorylation affects the...
  11. Answers
    2
    Views
    893

    I'm guessing that one of the options is nerve...

    I'm guessing that one of the options is nerve cells. They last a lifetime (unless you drink too much!).
  12. Question: Can't Decide.

    by medgen
    Answers
    7
    Views
    3,342

    Just to add my $.02, while what much of what...

    Just to add my $.02, while what much of what Fr_Chuck has to say is correct, it makes the job sound pretty boring...

    Personally, after spending many years training to become a research...
  13. Answers
    2
    Views
    1,848

    I have left Taq out for hours -- don't worry...

    I have left Taq out for hours -- don't worry about it! I think it has a half-life of two hours or so at 95 degrees or something like that, so at room temperature it will barely suffer at all. In...
  14. Answers
    1
    Views
    1,088

    Wow, who would have ever thought I would answer...

    Wow, who would have ever thought I would answer this one here. My PhD happened to be on the Signal Recognition Particle Receptor and we used this assay regularly!

    Basically, you target the...
  15. Answers
    6
    Views
    1,364

    Theoretically, Capuchin is correct (If you were...

    Theoretically, Capuchin is correct (If you were able to produce perfect lenses). Practically, most modern standard light microscopes will give you about 1000X magnification. You can easily see a...
  16. Answers
    1
    Views
    1,520

    My experience is that you are most likely to get...

    My experience is that you are most likely to get the job where you do your internship. Work 200% harder than you normally would and focus more on your internship than your schooling -- so long as...
  17. Question: Human VS Pig

    by medgen
    Answers
    7
    Views
    23,120

    OK, so if you 'mixed them together' then 'telling...

    OK, so if you 'mixed them together' then 'telling them apart' would be virtually impossible, since they are chemically very similar and at this point would be mixed. Something like FACS might...
  18. Answers
    60
    Views
    80,035

    I have to agree 100% with Tallarin. I got the...

    I have to agree 100% with Tallarin. I got the label (at about age 8) and spent a few years in a 'special' environment -- worst time of my life! Other than that, my parents were never too much into...
  19. Question: Null hypothesis

    by medgen
    Answers
    7
    Views
    3,037

    Important to note that this only holds if the...

    Important to note that this only holds if the hypotheses are mutually exclusive AND cover all the possible subsets. It is a common mistake made by novices to stats and other scientists.

    More...
  20. Answers
    5
    Views
    5,913

    Sovaira, I wasn't meaning to impugne your...

    Sovaira,

    I wasn't meaning to impugne your answer. Actually, I was giving my response to the original post. And by 'quick answer', I was referring to my quick answer. I'm sorry for the confusion...
  21. Question: Null hypothesis

    by medgen
    Answers
    7
    Views
    3,037

    The null hypothesis is usually set as the...

    The null hypothesis is usually set as the *opposite* of what you expect to prove. It is usually a statistical construct, but can be used in all aspects of the scientific method. Essentially, the...
  22. Answers
    5
    Views
    5,913

    Very quick answer -- not even close. Olive oil...

    Very quick answer -- not even close. Olive oil is composed almost exclusively of trigylcerides with unsaturated fatty acids. Blood is an incredibly complex mix of cells, plasma, proteins, fats,...
  23. Question: Black Lights

    by medgen
    Answers
    3
    Views
    6,362

    Very simply -- all alternate light sources (e.g....

    Very simply -- all alternate light sources (e.g. black lights) are pretty much useless. When we receive a submission where an investigator/nurse indicates that they used such a tool, we pretty much...
  24. Question: Enzyme activity

    by medgen
    Answers
    1
    Views
    2,788

    Um, yes -- but it is quite complicated. I'll try...

    Um, yes -- but it is quite complicated. I'll try to sum it up this way -- lock and key theory suggested that enzymes worked by producing a binding pocket that had a 3D shape that perfectly fit its...
  25. Question: What classes

    by medgen
    Answers
    3
    Views
    2,172

    Too late now I guess, but generally get a broad...

    Too late now I guess, but generally get a broad background in all the sciences. One that is often overlooked is stats. That is what it all comes down to and many forensic scienctists lack. ...
  26. Question: Blood Type

    by medgen
    Answers
    5
    Views
    2,423

    SOMEONE has something wrong here. If you are...

    SOMEONE has something wrong here. If you are both truly type O then your child must be type O. Some very rare things (like chimerism in you/your husband) could explain this, but it is almst unheard...
  27. Question: Real time PCR

    by medgen
    Answers
    2
    Views
    854

    1. No cloning is necessary -- you will be...

    1. No cloning is necessary -- you will be looking at mRNA. You only need to design appropriate primers

    2. TaqMan is by far the most specific and accurate method (Applied Biosystems). Sybr...
  28. Answers
    14
    Views
    3,096

    I do happen to be a forensic scientist. As for...

    I do happen to be a forensic scientist. As for the credentials, here goes: PhD in Biochemistry, Post-doctoral studies in Medical genetics. Two years as a practicing forensic scientist working in...
  29. I guess my only problem with this approach is...

    I guess my only problem with this approach is that I might then be liable for court fees, payment of their legal fees etc. which I imagine will be much more than the $950 they say we currently owe. ...
  30. Credit agency refuses to verify debt, but demands payment (Ontario)

    Hello,

    Just as a backgrounder, I have placed my original post in quotes at the bottom. The one thing I should add is that we contacted the original alleged creditor (GMAC), who was reasonably...
  31. Answers
    4
    Views
    3,542

    I am going to use the DEBT COLLECTOR DISCLOSURE...

    I am going to use the DEBT COLLECTOR DISCLOSURE STATEMENT in the sticky at the top of this forum to find out about the validity of the debt. Can anyone tell me if there is anything specific to...
  32. Answers
    4
    Views
    3,542

    Unknown debt problem -- Ontario, Canada

    NOTE: Prior to reading this, please note that I reside in Ontario, Canada. I would appreciate inpur from anyone with knowledge about our system.


    Hello,

    Myself and my wife have always prided...
Results 1 to 32 of 32