Ask Experts Questions for FREE Help !
Ask
    tulipkur's Avatar
    tulipkur Posts: 2, Reputation: 1
    New Member
    		  
    #1

    Oct 20, 2009, 03:35 PM
    Which microscope?
    What microscope would you use to view a gold-dipped specimen?
    a)TEM microscope
    2)SEM Microscope
    3)Both TEM and SEM
    4)Neither TEM or SEM
    Alty's Avatar
    Alty Posts: 28,317, Reputation: 5972
    Pets Expert
    		  
    #2

    Oct 20, 2009, 04:06 PM

    Which answer do you think is right and why?
    KISS's Avatar
    KISS Posts: 12,510, Reputation: 839
    Uber Member
    		  
    #3

    Oct 20, 2009, 07:31 PM

    Both SEM and TEM. The thin layer of gold prevents the sample from charging. That would work if the gold is sputtered.

    What bothers me with this question is the word "dipping". This bothers me a bit: SPI-Mark™ Colloidal Gold Reagents - Selection of the Right Conjugate - SPI Supplies

    That states that you need something else besides gold to make it stick.

    When you use the term DIP, I would probably change my answer to SEM only.

    What do you think?

    SEM's I've used and fixed. TEM's I have not.
    Perito's Avatar
    Perito Posts: 3,139, Reputation: 150
    Ultra Member
    		  
    #4

    Oct 20, 2009, 08:06 PM

    I agree with the "dipping" comment. When I used to do a lot of SEM work, we sputter-coated samples with gold and occasionally with other metals.

    With backscattered-electron detectors, you don't need to coat samples. Some TEM samples are also not coated.
Not your question? Ask your question View similar questions
 

Question Tools Search this Question
Search this Question:

Advanced Search


Check out some similar questions!

Using a microscope [ 2 Answers ]

If your using an up to daye light microscope to examine animals cells,you won't beable to detect what

Salive microscope [ 1 Answers ]

I've been trying to conceive after having a mc in June ,the other day I bought a saliva microscope and was wondering if they have really worked for anyone on here Any replies will be very helpful to me

Light microscope [ 1 Answers ]

If you're using an up-to-date light microscope to examine animal cells, you won't be able to detect____

Microscope detection [ 1 Answers ]

If you are using an up to date light microscope to examine animal cells you won't be able to detect what?

View more questions Search