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    setarehasadi's Avatar
    setarehasadi Posts: 2, Reputation: 1
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    #1

    Sep 22, 2007, 10:00 AM
    Why endonucleases use polindromic sequences?
    Hello I want to know why the cleavage site of endonucleases are polindromic? Thanks
    templelane's Avatar
    templelane Posts: 1,177, Reputation: 227
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    #2

    Sep 26, 2007, 01:10 PM
    They are not always palindromic
    BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5′-CACGTC(-3/-3)-3′

    However if the endonuclease does cleave at a palindromic site then if sticky ends are produced they will be compatible. Whereas if the sequence was not they wouldn't be. Although I can't think why this would have benefited the bacteria which initially evolved them as a defence mechanism against viruses. Good job they did though or science would be a very different place today!

    I've had a good look around the web and I can't find a satisfying answer! I'll ask in my lab if anyone knows tomorrow.
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