Question: DNA Melting
View Single Post
jem02081's Avatar
jem02081 Posts: 64, Reputation: 90
Junior Member
 
#2

Mar 21, 2009, 03:51 PM
Since this appears to be a homework question Ill just give you a couple of hints.
First, Region 1 is longer
Second, Region 2 has a much higher G+C content

Which has the higher Tm? Try googling oligo & tm. You will find quite a few Tm calculators which will answer this question

The answer to why depends how sophisticated of an answer you need. The question could be asked in high school or college. For a reasonably sophisticated answer see "DNA denaturation" as Wikipedia.
Helpful