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    avinash_m's Avatar
    avinash_m Posts: 2, Reputation: 1
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    #1

    Dec 8, 2009, 12:41 AM
    Why 1.2 % KCL used for stray light calibration in UV Vis Spectrophotometer
    Why 1.2 % KCL used for stray light calibration in UV Vis Spectrophotometer
    Perito's Avatar
    Perito Posts: 3,139, Reputation: 150
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    #2

    Dec 20, 2009, 11:16 AM

    "Stray light" is light from wavelengths other than the desired wavelength.

    A solution of 1.2% (I'm not exactly sure why it's always 1.2%) provides ionic strength to the solution (hence, it changes the refractive index so as to be similar to comparable solutions), but it doesn't absorb much in the UV or Visible region. Therefore, light hitting the detector will be almost all stray light.

    KCl cuts off at 200 nM.
    avinash_m's Avatar
    avinash_m Posts: 2, Reputation: 1
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    #3

    Jan 7, 2010, 05:56 AM
    Thanks
    dacktmal's Avatar
    dacktmal Posts: 1, Reputation: 1
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    #4

    Apr 30, 2012, 12:50 AM
    I think the limit for the stray light by using KCl is not 200 nm rather it is 198 nm now.
    Ernestobh's Avatar
    Ernestobh Posts: 1, Reputation: 1
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    #5

    Jun 13, 2012, 12:45 PM
    The Stray Light Reference Materials are useful in determining the amount of stray light in your instrument because each material stops transmitting light below a specific wavelength. Hence, below a specified "cur-off" wavelength, any indication of light transmission must be stray light.

    See: http://www.starnacells.com/d_ref/d_sl/Stray.html

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