I have Vo at units of umol min-1 mL-1 and substrate at unit of mM
do I need to manipulate the data of the Vo values? Or just leave them as they are as the conversion factor is 1?
Just my first value is 0.0022 for Vo and 1/0.0022 is 454 which doesn't seem right? Please help it'll be greatly appreciated.

It's hard to guess what you're doing exactly with the amount of information provided...

It's hard to guess what you're doing exactly with the amount of information provided...

This is for a lineweaver Burke plot. I have a table as follows:
Substrate (mM) No inhibitor. Inhibitor
Vo(umol min-1 mL-1)
0.05. 0.0022. 0.0045
0.1. 0.0033. 0.0083
0.2. 0.044. 0.0154
0.5. 0.059. 0.0286
2. 0.063. 0.05

I know these values will be the same when converted to mM-1 min. However 1/0.0022 is 454 which doesn't seem right ? Please can you tell me where I'm going wrong ? Thank you in advance !

Well, if you take those reciprocals as you obtained them and work out the Michaelis–Menten constant, do you get what you expected?

If yes, then you did it right. If not, then you know that something is wrong. :)