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Tazzy101
Feb 11, 2012, 06:51 AM
My question is, what effect does high temperatures have on the extraction of DNA from wheatgerm? Does lower temps increase the amount of DNA extracted?

jem02081
Feb 14, 2012, 08:18 PM
Unfortunately, the answer to your question is that it depends on the extraction method. With an extraction method like http://learn.genetics.utah.edu/archive/wheatgerm/index.html
I suspect the detergent you used would be biggest variable.
Higher temperatures in the extraction method would work to help free the DNA from the other constituents, I don't think you should worry about DNA stability until the temperature is close to boiling & then the worry is denaturation. I don't think that low temperatures would be better other than to protect the DNA from DNase, but I don't think that is a significant concern.