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View Full Version : How to know the content of beta carotene in a solution?


Harst
Dec 29, 2010, 07:59 PM
Hi all... is there any way to know the content of beta carotene in a solution?

Form of beta carotene is C40H56...

Whether there are elements that can react with beta-carotene in solution so that can change the color of the solution?

Is anyone here know about that? Help me please... thanks

DrBob1
Dec 29, 2010, 09:22 PM
There are many reagents that will react with the double bonds in beta-carotene. Many of them are colored (I2, Br2, KMnO4) and will be declolorized upon reaction. They also will decolorize the beta-carotene itself but I don't think this could be used quantitatively since the color of beta-carotene comes from its long conjugated system of double bonds. Fewer double bonds or an interrupted conjugated system would also change the color. I think you would be better served with UV/VIS spectroscopy. Hydrogenation to completion would be another possibility. You can also compare the color of known solutions with a solution of unknown concentration. Does this help?

Harst
Jan 10, 2011, 08:37 PM
Thanks a lot DrBob1 for the answer... which one is the cheapest between I2, Br2, KMnO4?