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View Full Version : List the function of the following parts of the light microscope


hslove142331
Jan 21, 2010, 09:16 PM
1.Ocular-(Eyepiece Lens) the lens at the top that you look through.
2.Objectives-Usually you will find 3 or 4 objective lenses on a microscope. They almost always consist of 4X, 10X, 40X and 100X powers. When coupled with a 10X (most common) eyepiece lens, we get total magnifications of 40X (4X times 10X), 100X , 400X and 1000X. To have good resolution at 1000X, you will need a relatively sophisticated microscope with an Abbe condenser. The shortest lens is the lowest power, the longest one is the lens with the greatest power. Lenses are color coded and if built to DIN standards are interchangeable between microscopes. The high power objective lenses are retractable (i.e. 40XR). This means that if they hit a slide, the end of the lens will push in (spring loaded) thereby protecting the lens and the slide. All quality microscopes have achromatic, parcentered, parfocal lenses.
3.Stage-The flat platform where you place your slides. Stage clips hold the slides in place. If your microscope has a mechanical stage, you will be able to move the slide around by turning two knobs. One moves it left and right, the other moves it up and down.
4.Condenser-
5.Iris diaphragm-Many microscopes have a rotating disk under the stage. This diaphragm has different sized holes and is used to vary the intensity and size of the cone of light that is projected upward into the slide. There is no set rule regarding which setting to use for a particular power. Rather, the setting is a function of the transparency of the specimen, the degree of contrast you desire and the particular objective lens in use.
6.coarse adjustment-near focus when erotated into position and will only need the fine adjustment for a sharp image
7.fine adjustment
8.light

I don't know three of them, condenser, fine adjustment, light.
Please help me what functions they are

KISS
Jan 21, 2010, 10:20 PM
See Condenser (microscope) - Wikipedia, the free encyclopedia (http://en.wikipedia.org/wiki/Condenser_%28microscope%29)

And the glossery of terms link at the bottom. Light is called something else.

Fine adjustment isn't covered. Normally microscopes have a fine and course focus contol, so it moves the stage by a smaller amount.

I'll also comment, that the light can be directed through the specimin at the bottom or at the specimin on top. The intensity is usually adjustible. You can add polarization and color to the light source as well.

KISS
Jan 22, 2010, 02:46 AM
Your stage answer is slightly wrong. Left and right and back and forth.

I believe fine and course focus adjustments move it up and down. Depending on the microscope, it doesn't have to.