View Full Version : Which microscope?
tulipkur
Oct 20, 2009, 03:35 PM
What microscope would you use to view a gold-dipped specimen?
a)TEM microscope
2)SEM Microscope
3)Both TEM and SEM
4)Neither TEM or SEM
Alty
Oct 20, 2009, 04:06 PM
Which answer do you think is right and why?
KISS
Oct 20, 2009, 07:31 PM
Both SEM and TEM. The thin layer of gold prevents the sample from charging. That would work if the gold is sputtered.
What bothers me with this question is the word "dipping". This bothers me a bit: SPI-Mark™ Colloidal Gold Reagents - Selection of the Right Conjugate - SPI Supplies (http://www.2spi.com/catalog/chem/SPI-Mark-Colloidal-Gold-Reagents-Selection-Sample-Types.html)
That states that you need something else besides gold to make it stick.
When you use the term DIP, I would probably change my answer to SEM only.
What do you think?
SEM's I've used and fixed. TEM's I have not.
Perito
Oct 20, 2009, 08:06 PM
I agree with the "dipping" comment. When I used to do a lot of SEM work, we sputter-coated samples with gold and occasionally with other metals.
With backscattered-electron detectors, you don't need to coat samples. Some TEM samples are also not coated.