I have an spreadsheet that I am working on. I have set up Macros buttons that when selected will changed the color(format) of the cell to correspond however once the cell is changed(formatted) I want to cell to lock automatically so that it can't be changed? Is this an available option for excel?

To do this will require more VBA.

First, unlock the cells you want editable
highlight all the cells
Press Ctrl-1 to open the format window
Click the Protection tab
Uncheck the [ ] Locked option
Add a sheet-level macro to lock cells as they are valued
Right-click on the sheet tab and select VIEW CODE
Paste in this macro:

Option Explicit
Const pw As String = "password"

Private Sub Worksheet_Change(ByVal Target As Range)
Dim cell As Range
Me.Unprotect pw

For Each cell In Target
If cell.Value <> "" Then cell.Locked = True
Next cell

Me.Protect pw
End Sub
Press Alt-Q to close the editor
Save your sheet

Of course, set your own protection password in that constant.

There are many many ways to do this, this just being one.