What is destop.ini?
Is it virus?

Not a virus... it's a harmless configuration file Windows uses to tell Windows Explorer how you want this particular folder to be displayed, if it is different from the global default settings.

If you really want to get rid of these file(s) anyway, here's a page explaining step by step:
How to get rid of Desktop.ini (http://www.ofzenandcomputing.com/zanswers/797)