I am replacing a white rodgers thermostat (with emergency heat setting) that is controlling in floor heating in the main level of my house. It is non programmable. I am replacing it with a totaline p374-1800. The WR has a connection that is "E" that is jumper wired to W2. The totaline doesn't have this connector. Do I need this jumper wire? Will it still work?

Really depends upon how the original sysyem is wired. For some reason the older thermostat sems to have two stage heat capability. You will need to trace the wiring out to see what you have.

((The totaline doesn't have this connector.))

What connector? The E or the W2?

I am replacing a white rodgers thermostat (with emergency heat setting) that is controlling in floor heating in the main level of my house. It is non programmable. I am replacing it with a totaline p374-1800. The WR has a connection that is "E" that is jumper wired to W2. The totaline doesn't have this connector. Do I need this jumper wire? Will it still work?
The totaline does't have the E connector. For whatever it may help someone, original WR is an 1F89-211 (heat pump T-stat) although I don't have a heat pump.
I believe the reason for this, was because it also originally controlled the forced air heat that heats the upstairs of the house (when the emergency heat kicked on). It didn't do it accurately, so the HVAC company installed a T-Stat upstairs to control the forced air ductwork (heating and cooling) while this WR downstairs now only controls the in floor heating.

Here is how the wiring was connected in the old WR:
Wire Connection
Blue C
Red R
Green G
Brown O-B
Yellow Y
Jumper wire W2 to E

If that is the case the jumper is not needed and W2 or E is not necessary.

If you are just using single stage heat.This is a diagram and instructions for your old thermostat.

With all the info below you will have all the wiring help anyone can give you for your system. As you will see without a heat pump O/B change over relay should not be necessary but you never know how they wired your system since it controls two systems at one time.

http://www.white-rodgers.com/wrdhom/pdfs/instruction_sheets/0037-6233.pdf

Wiring again

http://www.white-rodgers.com/wrdhom/pdfs/06_Cat_pages/Cat_06_pg0179.pdf

And more info

http://www.white-rodgers.com/wrdhom/pdfs/instruction_sheets/0037-6262.pdf