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View Full Version : Biochemistry standard curve for glucose


flick
Aug 1, 2007, 12:18 PM
How do u do the following haven't got a clue how to do these:confused

Glucose micrograms/ml absorbance 620nm
0 0.035; 0.028
10 0.085; 0.091
30 0.185; 0.191
60 0.335; 0.342
90 0.480; 0.478
1ml hydrolysate 0.275; 0.266

(I) plot these data and establish a standrad curve for glucose
(ii) determine the glucose content of the final hydrolysate
(iii) determine the glucose content of the original sample
(iv) establish the percentage hydrolysis of glycogen

templelane
Aug 1, 2007, 12:26 PM
Here is a partial hand to help you on your way

If you plot micrograms/ml of glucose (first column) against mg absorbance (second and third column- average these) on a graph. This is your standard curve.

Right I won't give you all the answers as once you have this the rest should become clear- post back if you are still stuck/ confused and I'll help you will the rest.