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    kikin azri's Avatar
    kikin azri Posts: 1, Reputation: 1
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    #1

    Jul 16, 2011, 05:41 PM
    Does the erythrocyte become haemolysed if the solution is still clear?
    How do you explain this situation?
    jcaron2's Avatar
    jcaron2 Posts: 986, Reputation: 204
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    #2

    Jul 18, 2011, 07:56 PM
    Still clear? I'm not an expert in this area, but if my memory serves me correctly, I'm pretty certain the solution starts cloudy (due to scattering of light by the healthy erythrocytes) and becomes clear if haemolysis takes place (i.e. the solution is hypotonic to the cells). The osmotic pressure into the cells causes them to lyse. This releases hemoglobin into the solution, turning it a deeper shade of red, but also resulting in clarification (since there are no longer whole cells to scatter the light). Since lysing is obviously irreversible, I can't see why you'd expect the solution to to ever change from being clear.

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