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Examination of a mammalian blood smear
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"Microscopic examination of whole blood begins with the preparation of a smear. A small drop of blood is placed at one end of a very clean glass slide, and the edge of a second slide is drawn across the drop at an angle so that capillary action spreads the drop along the edge. The second slide is then pushed in one smooth motion to the opposite end of the first slide, spreading the drop across the slide to make the smear."
"After the smear has dried it can be stained by applying a liberal amount of
Wright's stain with a pasteur pipet.
Wright's stain contains red and blue dyes that are acidophilic ('acid-loving') and basophilic ('alkaline-loving'), respectively. Avoid skin contact, since most stains are toxic. The stain is allowed to remain on the slide for about 2 minutes, and more is added if it begins to dry up. After a short dip in buffer and/or a rinse in deionized water, the smear is gently blotted dry with bibulous (absorbant) paper. Incidentally, many students have a problem with common sense - my apologies if this insults your intelligence, but DO remove the sheet of bibulous paper from the book before using it for blotting. Don't simply blot the slide onto the entire book."
Linear Mode
