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Home > Education > Research   »   Hydrolysis of RNA in Acid

 
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Old May 13, 2009, 04:35 AM
halley_fyd
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Hydrolysis of RNA in Acid



Can you please tell me how or what would be the reaction if you 10% of H2SO4on a test tube with RNA?

BENEDICTS'S TEST
On the first experiment, solid Na2CO3 will be place on the test tube with 10% of H2SO4 and RNA on it. Would you please tell me what would be the reaction that will happen?

TEST FOR PURINE
On the second experiment, 1% AgNO3 will be placed on a test tube that has the given 10% H2SO4 and RNA on it. What would be the reaction?

TEST FOR PENTOSE
On the third experiment, 5ml of Bial's reagent will be placed on a test tube with 10% of H2SO4 and RNA on it. What would be the reaction?

TEST FOR PHOSPHATE
On the fourth experiment, 5 drops of ammonia water that is acidify with 2 drops of 6N HNO3 and (NH4)2 MoO4, it will be placed on a hot water bath for minutes. What would be the reaction?


*The RNA used is the Isolated RNA from yeast.


Please also the rationales on why those things happen.

Thanks. I need it so badly.

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Old May 14, 2009, 06:23 AM   #2  
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what is the result of NA2CO3 with benedict's solution
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Old May 14, 2009, 10:00 AM   #3  
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guys, can you please answer this question? i also need it!
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Old Aug 28, 2009, 06:21 AM   #4  
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benedict's test: brick red ppt
orcinol test: bluish green sol'n
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