Ask Experts Questions for FREE Help!
Answer   ||    Advanced Search

Ask your question or search...
International Sites: Nederlandse experts vragen
User Name 
Password 
Join   Forgot password? 

Home > Home & Garden > Gardening & Plants   »   Viable Seeds

Question
 
 
#1  
Old Oct 28, 2009, 07:35 AM
phlanx's Avatar
phlanx
Full Member
phlanx is offline
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
Viable Seeds

Helllo

I have saved up my chilli seeds all year, air dried them and kept them in the fridge to slow the metabolic rate

I have noticed that some seeds I have used before germinate quicker than others, and the only difference is how long the seed has been in the fridge

Is there a point where the seed reaches its ideal state and therefore, when I am ready to seed it will germinate quicker than one that hasnt been in the cool for as long?

If so why?

Reply With Quote
 
     

Answers
 
 
Old Oct 28, 2009, 09:49 AM   #2  
Gardening Expert
KUXJ is offline
 
KUXJ's Avatar
 
Join Date: May 2009
Location: Central Pa.
Posts: 771
KUXJ See this member's comment history on his/her Profile page.KUXJ See this member's comment history on his/her Profile page.
Hi! phlanx

Borrowed from: chile pepper seed saving
"To maintain seed viability, they should be stored in a cool, dark, dry place preferably between 35-50°F ~ 1.6-10°C. One preferred method is to store seeds in clearly labelled water tight plastic envelopes in a Tupperware container at the bottom of the fridge. The cool, dark conditions mimic nature as the metabolism of the seeds slows down. Again, ensure seeds are sufficiently dried before storing them in the freezer as excess water/moisture can rupture the cell walls and your seeds will die!"

Moisture content should be approximately 8%, as a rule of thumb, seeds should break, not bend as you test them.

k
  Reply With Quote
 
     
 
 
Old Oct 28, 2009, 10:58 AM   #3  
Full Member
phlanx is offline
 
phlanx's Avatar
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
Cheers KUXj ,


This however I understand, but I was wondering of anyone knew of what nature does during this cycle

During winter, the temp drops and the seeds hibernate, this process occurs over Nov - Feb naturally

However, I am not leaving them long in the fridge, and I was wondering if there was a trigger within the DNA of the seed that told it is now in hibernation, so when it warms up with a little moisture it is now time to germinate

If this process does occur, is it simply the process of dropping the temp on the seed or the duration of time at that temp?
  Reply With Quote
 
     
 
 
Old Oct 28, 2009, 11:12 AM   #4  
Gardening Expert
KUXJ is offline
 
KUXJ's Avatar
 
Join Date: May 2009
Location: Central Pa.
Posts: 771
KUXJ See this member's comment history on his/her Profile page.KUXJ See this member's comment history on his/her Profile page.
Now that you've clarified your question, and I see from what direction your coming from, it will take a while longer to verify your theory, and a good one it is. One that I have no trouble accepting.

But we need qualitative assurance that, that, is actually happening. Unfortunately I'm going out to do chores now, and most likely will not be able to post back until later this evening. Say 2 or 3 am your time.

Cheers

k
  Reply With Quote
 
     
 
 
Old Oct 28, 2009, 12:16 PM   #5  
Full Member
phlanx is offline
 
phlanx's Avatar
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
No problem, K

I have been trying to find out myself from the internet and cant find it anywhere

I have spoken to a friend who manipulates plant dna - still no idea

The question partly came about from reading an article about Kew Gardens in England, they have achieved storing 10% of the worlds seeds, and they store them at -270c

Above this they state there is still metabolic reaction occuring, and that got me thinking as to why some seeds I have planted, have germinated quicker than others, while still being the same species and same environment

Anyway, thanks for helping
  Reply With Quote
 
     
 
 
Old Oct 28, 2009, 08:54 PM   #6  
Gardening Expert
KUXJ is offline
 
KUXJ's Avatar
 
Join Date: May 2009
Location: Central Pa.
Posts: 771
KUXJ See this member's comment history on his/her Profile page.KUXJ See this member's comment history on his/her Profile page.
Quote:
Originally Posted by phlanx View Post
No problem, K

I have been trying to find out myself from the internet and cant find it anywhere

I have spoken to a friend who manipulates plant dna - still no idea

The question partly came about from reading an article about Kew Gardens in England, they have achieved storing 10% of the worlds seeds, and they store them at -270c

Above this they state there is still metabolic reaction occuring, and that got me thinking as to why some seeds I have planted, have germinated quicker than others, while still being the same species and same environment

Anyway, thanks for helping
Hi! phlanx, your welcome.

The Kew is an excellent starting source for study material..
Royal Botanic Gardens, Kew - Home Page

Try thinking about your question this way......

After seed has been prepared for storage it is usually placed in a cool, dark environment, this accomplishes two things:
1.) It is common knowledge that cold slows down the metabolic rate of organic material, which also includes the seeds genome.
Freezing seed overwinter also has a bearing on destroying fungal and parasitic diseases.

2.) Darkness keeps the genetic switch for growth turned off.

So these two factors, and your conscientious selection of seed from your garden is what determines your success rate of propagation.

Even though you state:
Quote:
while still being the same species and same environment
Unbeknownst to you, and this is very easy to do in an non-laboratory setting, you may be introducing variants in the seed selection, and storage process.
It is physically impossible for the average homeowner to watch over his or her dominion 24/7 to maintain consistency in the growing process.
  • Do any of your plants show any signs of stress or disease?
  • Are your tools, hands, and work area disinfected/kept sterile during the seed selection, and storage process?
  • Once the seeds are prepared, are there any inconsistencies In the storage process? The simple act of the light turning on when you open the refrigerator door is enough to disrupt the process. Dark means dark.
  • Any variation in temperature is a disruption, once the temp has been reached it should be maintained or inconsistencies will emerge.

I have many sites in my chest o' marks that have conducted studies on the genetics of seed dormancy, but unfortunately chili seed genes, do not make for a good study.

The studies are conducted on variants of genomes (simple structure) that are similar to the major structural groups, and then extrapolated to a specific target group.

I offer several links pertaining to seed dormancy for your further study, and I will post back if I come across any more studies pertinent to your topic.
It is an interesting subject.

“Life is much simpler at the molecular level.”

Cheers

k

USDA study on seed dormancy showing QTLs affect on preharvest sprouting :
ARS | Publication request: Genetics of Seed Dormancy in Weedy Rice

QTL:
Quantitative trait locus - Wikipedia

The genetics of seed quality in Arabidopsis thaliana a rather large 136page report:
http://library.wur.nl/wda/dissertations/dis3525.pdf
ABRC Home Page

Arabidopsis thaliana:
Arabidopsis - Wikipedia

Links to Seeds and Plant breeding:
Seeds and Plant Breeding : Alternative Crops and Plants : Alternative Farming Systems Information Center

And going back to thechileman, see soil temperature:
chile pepper starting seeds
  Reply With Quote
 
     
 
 
Old Oct 29, 2009, 04:58 AM   #7  
Full Member
phlanx is offline
 
phlanx's Avatar
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
Morning K

Thanks for that, really appreciate it

I understand that home growing is never going to be accurate as you cannot control the environment

I will have a read of the aticules later when I have an hour

I guess what it comes down to to as yuo say is a veriation of preperation even though I cannot stop that, and good old fashioned mother natur producing a variation of the seeds, the thickness of the seed coat as one particular example

Thanks again K
  Reply With Quote
 
     
 
 
Old Oct 30, 2009, 06:04 AM   #8  
Gardening Expert
KUXJ is offline
 
KUXJ's Avatar
 
Join Date: May 2009
Location: Central Pa.
Posts: 771
KUXJ See this member's comment history on his/her Profile page.KUXJ See this member's comment history on his/her Profile page.
Good Afternoon phlanx

Take a look at this:
Major flowering time gene, FLOWERING LOCUS C, regulates seed germination in Arabidopsis thaliana ? PNAS

k

Comments on this post
phlanx agrees: Superb material supplied to answer my question!
  Reply With Quote
 
     
 
 
Old Oct 30, 2009, 07:13 AM   #9  
Full Member
phlanx is offline
 
phlanx's Avatar
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
Salvo K

Okay then - thanks for that

It is times like this I wished I had listen in school

From what I understand does this sound right?

The FLC controls the plants cycle, so when a tempreture dependant plant feels the cold, the FLC flow will either increase or decrease which will trigger the genes which triggers a change in the cycle of the plant, so in this case tell it to go dormant for the winter

Now this same process also effects the make up of the seed, which makes sense as the seed is nothing more than just a really tiny plant

However, the maternal plant will pass on the DNA to the seed, but then the FLC comes along and effects the gene structure

So in essence each seed or fruit can be slightly different to the next one, which enables the plant to evolve and not stay stagnant in its devolopment

Is this right - if so wow, you got to love mother nature
  Reply With Quote
 
     
 
 
Old Oct 30, 2009, 07:37 AM   #10  
Full Member
phlanx is offline
 
phlanx's Avatar
 
Join Date: Oct 2009
Location: England
Posts: 212
phlanx See this member's comment history on his/her Profile page.
In addition

I knew that each seed would be slightly different from the next, seed coating for example is one prime reason for pregermination process

However, would you say it is the FLC that is effecting the seed genes while it grows and matures or would you say it is the FLC effecting the maternal genes which is then efefcting what part of the plants dna is dominant and therefore effect the seed

So to clarify (for myself mainly) The FLC is being effected by the environment of the plant, this in turn will always effect the certain genes in the DNA and depending on when the seeds form and mature is purely down to ultimatly the environment

Therefore, unless you can achive the perfect state of environment there is no way you a will get a complete set of seeds that match, and even then the FLC flow will not be totally constant and therefore the natural selection of evolution is maintained

Does this make sense or am I heading off to left field?
  Reply With Quote
 
     

Your Answer
Email me when someone replies to my answer
Join Login



Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes
Ask your question or search...



Similar Threads
poppy seeds
(1 replies)
Is Using Twitter a Viable Way to Advertise a Business, Service or To Sell Things?
(2 replies)
Is there room for viable third and fourth political parties ?
(8 replies)
Can Pomegrante seeds
(3 replies)
Is the Project Viable - Finance
(1 replies)

Thread Tools
Show Printable Version Show Printable Version
Email this Page Email this Page
Search this Thread

Advanced Search

Bookmarks





Copyright ©2003 - 2009, Ask Me Help Desk.
All times are GMT -8. The time now is 10:58 AM.