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Calculate concentration
Asked Oct 9, 2007, 12:52 PM
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1 Answer Hello, I have 38.8ug/ml (u = micrograms, don't know how to get the symbol) DNA in solution. Two questions: 1) If I add 1.4ul of this solution to 18.6ul of solution what is the formula to calculate the concentration of DNA in my new 20ul solution? 2) If I want 100ng of DNA in the new solution, how to calculate how much of the 38.8ug/ml DNA do I add, with a final volume of 20microliters? Thank you for your help. |
1 Answer
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| Volume1 x Concentration1 = Volume2 x Concentration2 V1 x C1 = V2 x C2 So Q1) 1.4ul X 38.8ug = (20ul) x ? Rearrange Remember in these kind of questions to make sure all the units are the same - not a problem but will be required for Q2. It's the same equation for the second qustion as well. | ||||
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