can anyone tell me what is a useful solution for precipitation of a protein in alkali and a useful solution for precipitation of a protein in an acid solution

templelane

Hi me again,

Anything it doesn't like and the stupid thing will precipitate. Anything away from the pI [1] (isoelectric point) will do the trick- which seems to be what you are after. In fact it can be pretty hard to keep a protein IN solution (sorry sore point). Just alter the pH of the solution your protein is dissolved in with something like NaOH, or HCl - or in fact anything that is a acid or a base should do it.

What is this for? What level are you studing at/what course? Just seems like a pretty random question, I'm not sure you are understanding your course very well. Perhaps you should speak to an advisor/teacher/lecturer to see where you can get some help.

I can try to help on here but nothing beats face to face tuition. Whatever you do don't leave things until right before the exams or it'll be a nightmare.

Good luck- hope you are getting on alright with your glucose curve question.



1) Isoelectric point - Wikipedia, the free encyclopedia
Protein Precipitation

spike g

Precipitation is widely used in downstream processing of biological products, such as proteins. [1] This unit operation serves to concentrate and fractionate the target product from various contaminants. For example, in the biotechnology industry protein precipitation is used to eliminate contaminants commonly contained in blood. [2] Academic research on protein precipitation explores new protein precipitation methods. [3] The underlying mechanism of precipitation is to alter the solvation potential of the solvent and thus lower the solubility of the solute by addition of a reagent.

Acetone can precipate a protein in a solution or an addition of N2 to an aliquot to dry out the protein.